m tb erdman strain  (ATCC)

Journal: Frontiers in Immunology

Article Title: Exosome-mediated bidirectional immune dysregulation in tuberculosis: proteomic profiling reveals strain-specific strategies of virulent H37Rv and attenuated H37Ra

doi: 10.3389/fimmu.2025.1696299

Figure Lengend Snippet: Schematic of the experimental workflow. THP-1 cells differentiate into macrophages (via PMA) and are exposed to Mtb (uninfected/NC, avirulent H37Ra/RA, virulent H37Rv/RV). Macrophages undergo bacterial load/cell viability assays; exosomes undergo morphology/particle/marker protein characterization. Macrophages and released exosomes are analyzed via proteomics (extraction→trypsin digestion→iTRAQ labeling→HPLC separation→TripleTOF 6600 mass spec→bioinformatics). Host/bacterial proteins are analyzed for differentially expressed genes, enriched pathways and functions to explore virulence - dependent immune mechanisms.

Article Snippet: Mycobacterium tuberculosis H37Rv (ATCC 27294) and H37Ra (ATCC 25177) were cultured in Middlebrook 7H9 broth (BD 271310) containing 10% OADC enrichment (BD 212351), 0.05% Tween 80 (Sigma-Aldrich P1754), and 0.2% glycerol (Sigma-Aldrich G5516) to mid-log phase (OD 600 =0.6-0.8).

Techniques: Marker, Extraction, Labeling

Journal: Frontiers in Immunology

Article Title: Exosome-mediated bidirectional immune dysregulation in tuberculosis: proteomic profiling reveals strain-specific strategies of virulent H37Rv and attenuated H37Ra

doi: 10.3389/fimmu.2025.1696299

Figure Lengend Snippet: Proteomic profiling of host differentially expressed proteins (DEPs) in macrophages and exosomes. (A) Heatmap of DEPs in macrophages showing the expressions of the DEPs in macrophages from three groups (|Fold change| ≥ 1.2, p ≤ 0.05). The color key indicates the expression level of the proteins. (B) The bar plots showed the numbers of DEPs in H37Ra (RA) and H37Rv (RV) infected macrophages compared with uninfected NC. Red bars indicated the number of up-regulated proteins in each sample, while the blue bars indicated the number of down-regulated proteins in each sample. (C) Heatmap of DEPs in exosomes from three groups (|Fold change| ≥ 1.2, p ≤ 0.05). The color key indicates the expression level of the proteins. (D) The bar plots showed the numbers of DEPs in exosomes of RA and RV infected macrophages compared with those of uninfected NC. Red bars indicated the number of up-regulated proteins in each sample, while the blue bars indicated the number of down-regulated proteins in each sample. (E) Venn diagram of RA/NC DEPs shared between macrophages and exosomes. Among the shared 27 proteins, 24 (88.9%) showed inverse regulation (up in cells, down in exosomes or vice versa). (F) Venn diagram of RV/NC DEPs shared between macrophages and exosomes. Only 29 DEPs overlapped, with 23 (79.3%) being inversely regulated.

Article Snippet: Mycobacterium tuberculosis H37Rv (ATCC 27294) and H37Ra (ATCC 25177) were cultured in Middlebrook 7H9 broth (BD 271310) containing 10% OADC enrichment (BD 212351), 0.05% Tween 80 (Sigma-Aldrich P1754), and 0.2% glycerol (Sigma-Aldrich G5516) to mid-log phase (OD 600 =0.6-0.8).

Techniques: Expressing, Infection

Journal: Frontiers in Immunology

Article Title: Exosome-mediated bidirectional immune dysregulation in tuberculosis: proteomic profiling reveals strain-specific strategies of virulent H37Rv and attenuated H37Ra

doi: 10.3389/fimmu.2025.1696299

Figure Lengend Snippet: Proposed model of exosome-mediated bidirectional immune dysregulation by Mtb strains H37Rv and H37Ra. This schematic summarizes the strain-specific strategies through which Mtb modulates host immunity via exosomal communication.

Article Snippet: Mycobacterium tuberculosis H37Rv (ATCC 27294) and H37Ra (ATCC 25177) were cultured in Middlebrook 7H9 broth (BD 271310) containing 10% OADC enrichment (BD 212351), 0.05% Tween 80 (Sigma-Aldrich P1754), and 0.2% glycerol (Sigma-Aldrich G5516) to mid-log phase (OD 600 =0.6-0.8).

Techniques: